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rapid dna ligation kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher rapid dna ligation kit
    Rapid Dna Ligation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rapid+dna+ligation+kit/Deoxyribonucleic+acid/pmc12869678-55-0-5
    Average 99 stars, based on 1 article reviews
    rapid dna ligation kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Multiomic data analyses unveiled a novel phenol-soluble modulin that induces trapping of extracellular lipases at the surface of Staphylococcus aureus cells.
    Article Snippet: Plasmids were purified from overnight cultures with the NZYMiniprep kit (NZYTech) and verified by Sanger sequenc ing. .. When required, DNA fragments were excised using FastDigest restriction enzymes (Thermo Scientific) and ligated into the appropriate vector with the Rapid DNA ligation kit (Thermo Scientific). ..

    Article Title: Design and Initial Characterization of a Novel Mini-Promoter for Gene Therapies Targeting the Central Nervous System
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Multiomic data analyses unveiled a novel phenol-soluble modulin that induces trapping of extracellular lipases at the surface of Staphylococcus aureus cells
    Article Snippet: Plasmids were purified from overnight cultures with the NZYMiniprep kit (NZYTech) and verified by Sanger sequencing. .. When required, DNA fragments were excised using FastDigest restriction enzymes (Thermo Scientific) and ligated into the appropriate vector with the Rapid DNA ligation kit (Thermo Scientific). ..

    DNA Ligation:

    Article Title: Multiomic data analyses unveiled a novel phenol-soluble modulin that induces trapping of extracellular lipases at the surface of Staphylococcus aureus cells.
    Article Snippet: Plasmids were purified from overnight cultures with the NZYMiniprep kit (NZYTech) and verified by Sanger sequenc ing. .. When required, DNA fragments were excised using FastDigest restriction enzymes (Thermo Scientific) and ligated into the appropriate vector with the Rapid DNA ligation kit (Thermo Scientific). ..

    Article Title: Mutant polymerases and methods of using the same
    Article Snippet: After template DNA digestion, PCR product of approximately 6.8 kbp was purified from 1% agarose gel using GeneJET Gel Extraction Kit (Thermo Scientific, K0691) according to the standard protocol. .. Circularization of mutated PCR products was performed by ligation with T4 DNA Ligase using Rapid DNA Ligation Kit (Thermo Scientific, K1423) according to the standard protocol (30 ng of mutated PCR product was used for each reaction). ..

    Article Title: A 5′-UTR cis -acting RNA element targeted by RNase III is essential for DNA simple sequence repeat-dependent phase variation in Haemophilus influenzae
    Article Snippet: .. Gel purification kit (Macherey-Nagel, 22740609.250) following the manufacturer instructions, and appropriate FastDigest restriction enzymes and Rapid DNA ligation kit (Thermo Fisher Scientific, K1423) were used. .. Escherichia coli TOP10 was used as an intermediate host, plasmids were extracted and purified (Qiagen, 12143), and transformed into H. influenzae electrocompetent cells [ ].

    Article Title: Single cell transcriptional perturbome in pluripotent stem cell models
    Article Snippet: FastDigest SgsI (AscI) , Thermo Scientific , #FD1894. .. Rapid DNA Ligation kit , Invitrogen , #K1423. .. SPRIselect beads , Beckman Coulter , # B23318.

    Article Title: Design and Initial Characterization of a Novel Mini-Promoter for Gene Therapies Targeting the Central Nervous System
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Multiomic data analyses unveiled a novel phenol-soluble modulin that induces trapping of extracellular lipases at the surface of Staphylococcus aureus cells
    Article Snippet: Plasmids were purified from overnight cultures with the NZYMiniprep kit (NZYTech) and verified by Sanger sequencing. .. When required, DNA fragments were excised using FastDigest restriction enzymes (Thermo Scientific) and ligated into the appropriate vector with the Rapid DNA ligation kit (Thermo Scientific). ..


    Article Title: Expanding the genetic landscape of inherited metabolic diseases using long-read sequencing and transcriptomic profiling.
    Article Snippet: The insert was excised with EcoRI (Roche Applied Science), purified using the QIAquick Gel Extraction Kit (Qiagen), and subsequently cloned into the pSPL3 vector dephosphorylated with Thermosensitive Alkaline Phosphatase (Promega). .. Ligation was performed using the Rapid DNA Ligation Kit (Thermo Fisher Scientific). ..

    Polymerase Chain Reaction:

    Article Title: Mutant polymerases and methods of using the same
    Article Snippet: After template DNA digestion, PCR product of approximately 6.8 kbp was purified from 1% agarose gel using GeneJET Gel Extraction Kit (Thermo Scientific, K0691) according to the standard protocol. .. Circularization of mutated PCR products was performed by ligation with T4 DNA Ligase using Rapid DNA Ligation Kit (Thermo Scientific, K1423) according to the standard protocol (30 ng of mutated PCR product was used for each reaction). ..

    Article Title: Design and Initial Characterization of a Novel Mini-Promoter for Gene Therapies Targeting the Central Nervous System
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Ligation:

    Article Title: Mutant polymerases and methods of using the same
    Article Snippet: After template DNA digestion, PCR product of approximately 6.8 kbp was purified from 1% agarose gel using GeneJET Gel Extraction Kit (Thermo Scientific, K0691) according to the standard protocol. .. Circularization of mutated PCR products was performed by ligation with T4 DNA Ligase using Rapid DNA Ligation Kit (Thermo Scientific, K1423) according to the standard protocol (30 ng of mutated PCR product was used for each reaction). ..

    Article Title: Expanding the genetic landscape of inherited metabolic diseases using long-read sequencing and transcriptomic profiling.
    Article Snippet: The insert was excised with EcoRI (Roche Applied Science), purified using the QIAquick Gel Extraction Kit (Qiagen), and subsequently cloned into the pSPL3 vector dephosphorylated with Thermosensitive Alkaline Phosphatase (Promega). .. Ligation was performed using the Rapid DNA Ligation Kit (Thermo Fisher Scientific). ..

    Gel Purification:

    Article Title: A 5′-UTR cis -acting RNA element targeted by RNase III is essential for DNA simple sequence repeat-dependent phase variation in Haemophilus influenzae
    Article Snippet: .. Gel purification kit (Macherey-Nagel, 22740609.250) following the manufacturer instructions, and appropriate FastDigest restriction enzymes and Rapid DNA ligation kit (Thermo Fisher Scientific, K1423) were used. .. Escherichia coli TOP10 was used as an intermediate host, plasmids were extracted and purified (Qiagen, 12143), and transformed into H. influenzae electrocompetent cells [ ].

    Purification:

    Article Title: Design and Initial Characterization of a Novel Mini-Promoter for Gene Therapies Targeting the Central Nervous System
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Clone Assay:

    Article Title: Design and Initial Characterization of a Novel Mini-Promoter for Gene Therapies Targeting the Central Nervous System
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.



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